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recombinant plasmids pet28a spytag mkate2  (Addgene inc)


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    Structured Review

    Addgene inc recombinant plasmids pet28a spytag mkate2
    Recombinant Plasmids Pet28a Spytag Mkate2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spytag003+mkate2/SpyTag003-mKate2+(Plasmid+%23133452)/pm40992952-318-12-15
    Average 93 stars, based on 11 article reviews
    recombinant plasmids pet28a spytag mkate2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Ionic Liquid-Induced Modulation of Ubiquitin Stability: The Dominant Role of Hydrophobic Interactions
    Article Snippet: .. PCR was performed using a high-fidelity DNA polymerase, PlatinumTM SuperFi II Master Mix (Invitrogen), following the manufacturer’s instructions. (Ubq) 9 gene was amplified by PCR from an existing plasmid (A kind gift from Prof. Julio Fernandez, pQE80L vector) and cloned into a plasmid obtained from Addgene, Spytag003-mkate2 (Addgene Plasmid #133452). ..

    Amplification:

    Article Title: Ionic Liquid-Induced Modulation of Ubiquitin Stability: The Dominant Role of Hydrophobic Interactions
    Article Snippet: .. PCR was performed using a high-fidelity DNA polymerase, PlatinumTM SuperFi II Master Mix (Invitrogen), following the manufacturer’s instructions. (Ubq) 9 gene was amplified by PCR from an existing plasmid (A kind gift from Prof. Julio Fernandez, pQE80L vector) and cloned into a plasmid obtained from Addgene, Spytag003-mkate2 (Addgene Plasmid #133452). ..

    Plasmid Preparation:

    Article Title: Ionic Liquid-Induced Modulation of Ubiquitin Stability: The Dominant Role of Hydrophobic Interactions
    Article Snippet: .. PCR was performed using a high-fidelity DNA polymerase, PlatinumTM SuperFi II Master Mix (Invitrogen), following the manufacturer’s instructions. (Ubq) 9 gene was amplified by PCR from an existing plasmid (A kind gift from Prof. Julio Fernandez, pQE80L vector) and cloned into a plasmid obtained from Addgene, Spytag003-mkate2 (Addgene Plasmid #133452). ..

    Clone Assay:

    Article Title: Ionic Liquid-Induced Modulation of Ubiquitin Stability: The Dominant Role of Hydrophobic Interactions
    Article Snippet: .. PCR was performed using a high-fidelity DNA polymerase, PlatinumTM SuperFi II Master Mix (Invitrogen), following the manufacturer’s instructions. (Ubq) 9 gene was amplified by PCR from an existing plasmid (A kind gift from Prof. Julio Fernandez, pQE80L vector) and cloned into a plasmid obtained from Addgene, Spytag003-mkate2 (Addgene Plasmid #133452). ..

    Purification:

    Article Title: Wafer‐Scale Integration of α‐quartz Thin Films toward Super High Frequency Piezoelectric bioNEMS for Chikungunya Virus Detection
    Article Snippet: .. SpyCatcher and SpyTag Purification: SpyTag003-mKate2 (Addgene ID 133 452) and SpyCatcher003-sfGFP (Addgene ID 133 449) were a gift from Mark Howarth, previously reported. .. [28] Briefly, to purify SpyTag-mKate2 and SpyCatcher-GFP, E. coli BL21 CodonPlus (DE3) RIPL (Agilent Technologies) cells were transformed with plasmid pET28a-SpyTag003-mKate2 or pJ404-SpyCatcher003-sfGFP.



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